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KMID : 0604520170430040309
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Journal of the Society of Cosmetic Scientists of Korea
2017 Volume.43 No. 4 p.309 ~ p.320
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Antioxidative Effects and Component Analysis of Graviola (Annona muricata) Leaf Extract/Fractions
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Park So-Hyun
Kim Ji-Min
Kim Jin-Hwan
Oh Yoon-Soo
Joo Dong-Hee
Lee Eun-Young
Shin Hyuk-Soo
Kim A-Rang
Lee Sang-Rae
Park Soo-Nam
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Abstract
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In this study, the antioxidative effects and component analysis of 50% ethanol extract, ethyl acetate fraction and aglycone fraction obtained from Annona muricata leaf were investigated. Free radical scavenging activities were performed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, total antioxidant capacities were estimated using luminol-dependent chemiluminescence assay and 1O2 quenching effects were estimated. Free radical scavenging activities (FSC50) of 50% ethanol extract, ethyl acetate fraction and aglycone fraction were 45.6, 29.8 and 18.0 μg/mL, and total antioxidant capacities (OSC50) were 4.4, 1.1 and 2.8 μg/mL, respectively. As a result of 1O2 quenching experiment, ethyl acetate and aglycone fraction showed similar activities to L-ascorbic acid used as a comparative control. The cellular protective effects of 50% ethanol extract on the 1O2-induced cellular damage of human erythrocytes were exhibited at concentration-dependent (5-50 μg/mL). TLC and HPLC were used to analyse components in the ethyl acetate fraction and aglycone fraction of Annona muricata leaf. In ethyl acetate fraction, rutin (quercetin-3-O-rutinoside), kaempferol- 3-O-neohesperidoside, nicotiflorin (kaempferol-3-O-rutinoside), p-coumaric acid were identified. In aglycone fraction, kaempferol was identified. These results suggest that the extracts of Annona muricata leaf have the applicability as antioxidant cosmeceutical ingredients.
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KEYWORD
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Annona muricata, antioxidative activity, cellular protective effect, component analysis, reactive oxygen species
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